Purification and characterization of an erythromycin esterase from an erythromycin-resistant Pseudomonas sp.
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Purification and Characterization of a Thermostable Neutrophilic Metalloprotease from Pseudomonas sp. DR89
A novel neutrophilic metalloprotease was isolated from Pseudomonas sp. DR89 isolate which was identified ina mineral spring in Iran. The enzyme was purified from the isolate to 21-folds in a three-step procedure involving ammonium sulfate precipitation, Q-Sepharose ionic exchange and Sephadex G-100 gel filtrationchromatography. Resuts showed that the enzyme was active at high temper...
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Background: The aim of this study was to investigate the frequency and type of bacteria resistant to chloramphenicol (CHL), erythromycin (E), and sulfamethoxazole (SXT) antibiotics from untreated wastewater effluents to receiving river. Methods: In total, 32 samples were taken from eight sites located in the raw wastewater to the downstream of the receiving Ghotor river in Khoy city. Resistant...
متن کاملPurification and characterization of an aldehyde oxidase from Pseudomonas sp. KY 4690.
An aldehyde oxidase, which oxidizes various aliphatic and aromatic aldehydes using O(2) as an electron acceptor, was purified from the cell-free extracts of Pseudomonas sp. KY 4690, a soil isolate, to an electrophoretically homogeneous state. The purified enzyme had a molecular mass of 132 kDa and consisted of three non-identical subunits with molecular masses of 88, 39, and 18 kDa. The absorpt...
متن کامل4-N-trimethylaminobutyraldehyde dehydrogenase: purification and characterization of an enzyme from Pseudomonas sp. 13CM.
4-N-trimethylaminobutyraldehyde dehydrogenase from Pseudomonas sp. 13CM was purified 14-fold to apparent homogeneity by hydrophobic chromatography on a Phenyl-Toyopearl, and affinity chromatography was done on a 5'-AMP Sepharose4B in the presence of dithiothreitol. The enzyme was found to be a trimer with identical 55 kDa subunits. The isoeletric point was found to be 5.5. The optimum temperatu...
متن کاملProduction, Partial Purification and Characterization of an Extracellular Psychrotrophic Lipase from Pseudomonas Sp. ADT3
Psychrotrophic Pseudomonas ADT3 (NCBI GenBank Acc.no.JX914667) is capable of growth on lipid as the sole carbon source. In this paper, we report the purification and characterization of an extracellular lipase from psychrotroph, isolated from soil sample of NyAlesund, Svalbard, Arctic region. The Pseudomonas ADT3 isolate produces lipase enzyme in the extracellular minimal media with only 1% oli...
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ژورنال
عنوان ژورنال: FEMS Microbiology Letters
سال: 2002
ISSN: 0378-1097
DOI: 10.1016/s0378-1097(02)00611-0